Separation of neutral proteins on ion-exchange resins
نویسندگان
چکیده
منابع مشابه
The chromatographic separation and purification of acidic proteins on carboxylated ion exchange resins.
The chromatographic separation of proteins on carboxylated ion exchange resins has been widely employed (1, 2). However, as pointed out previously by several authors (l-6), only proteins with alkaline or neutral isoelectric points have been investigated. This report deals with a systematic study of the behavior of proteins with acid isoelectric points on weak cation exchange resins.l In view of...
متن کاملDisplacement chromatography on synthetic ion-exchange resins. 1. Separation of organic bases and amino-acids using cation-exchange resins.
9. Accumulation of glutamate also occurred in isolated kidney cortex, spleen, lung and chorion. Six other tissues examined maintained a concentration gradient between tissue and medium, but did not increase the glutamate concentration above the original level. 10. It is pointed out that tissue slices afford a system in which problems of permeability and storage can be studied under controlled c...
متن کامل[Ion exchange resins].
Ion exchange resins have been used to help formulate pharmaceuticals since the late 1950's. During that time they have proved to be safe and effective excipients and are now used in many commercial formulations throughout the world. In this article we will look at some of the common problems faced by formulators and how using ion exchange resins may be able to solve them. Ion exchange resins ar...
متن کاملDisplacement Chromatography on Synthetic Ion - exchange Resins
In Part 1 of this series of papers (Partridge & Westall, 1949) it was shown that, whilst the baseexchange resin Zeo Karb 215 may be used with success for the separation of the neutral and acidic amino-acids, certain difficulties arise when the separation of basic substances is attempted. These difficulties were traced to the phenolic hydroxyl groups of the resin. Resins of the sulphonated pheno...
متن کاملKinetics study on separation of cadmium from tellurium in acidic solution media using ion-exchange resins.
The feasibility of using ion-exchange resins to separate cadmium from tellurium in acidic solutions of the two metals was investigated. We studied the competitive adsorption of cadmium and tellurium in such resins under varying acid strengths and contact time. We found that low sulfuric acid strength (i.e., 0.5 M) was most effective in removing cadmium from solutions. Different ion-exchange res...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1955
ISSN: 0306-3283
DOI: 10.1042/bj0590543